lucigen 30243-2 30281-2NxGen? T4 DNA Ligase RNAse-激情視頻網(wǎng)站guankan,日韓av在線一區(qū)二區(qū)三區(qū)Inhibitor

產(chǎn)品簡(jiǎn)介：北京中北林格供應(yīng)lucigen 30243-2 30281-2NxGen? T4 DNA Ligase RNAse-激情視頻網(wǎng)站guankan,日韓av在線一區(qū)二區(qū)三區(qū)Inhibitor，中北林格是lucigen總代理，提供lucigen產(chǎn)品貨號(hào)報(bào)價(jià)查詢(xún)。代理的其他品牌包括Biosearchtechnologies總代理，epicentre總代理，illumina總代理，ICLLAB總代理，ImmunoReagents總代理。</p>

產(chǎn)品名稱(chēng)：NxGen? T4 DNA Ligase (HighConcentration)</p>

貨號(hào)：lucigen30243-2</p>

規(guī)格：7,500 U</p>

存儲(chǔ)溫度：-20o C</p>

 

品牌：lucigen</p>

供應(yīng)商：中北林格</p>

產(chǎn)地：us</p>

發(fā)貨地：北京</p>

lucigen 30243-2 30281-2NxGen? T4 DNA Ligase RNAse-激情視頻網(wǎng)站guankan,日韓av在線一區(qū)二區(qū)三區(qū) Inhibitor 產(chǎn)品說(shuō)明書(shū)

Reliably join double-stranded nucleic acidchains.
可靠地連接雙鏈核酸鏈。

T4 DNA Ligase catalyzes the formation of a phosphodiester bondbetween the terminal 5′ phosphate and 3′ hydroxyl groups of duplexDNA or RNA. The enzyme efficiently joins blunt and cohesive endsand repairs single stranded nicks in duplex DNA, RNA or DNA/RNAhybrids.

T4DNA連接酶催化雙鏈DNA或RNA的末端5'磷酸和3'羥基之間的磷酸二酯鍵的形成。該酶有效地連接鈍性和粘性末端并修復(fù)雙鏈DNA，RNA或DNA/ RNA雜交體中的單鏈缺口。

T4 DNA Ligase VersionSizesLigase ConcentrationProvided BufferLowConcentration Ligase

1,500 U
7,500 U

2 U/μL10X T4 DNA Ligase BufferHigh Concentration Rapid Kit1,500U
7,500 U10 U/μL2X Rapid Ligation Buffer,
10X T4 DNA Ligase Buffer

T4 DNA連接酶版本尺寸連接酶濃度提供緩沖區(qū)低濃度連接酶

1,500 U 
7,500 U.

2 U /μL10X T4 DNA連接酶緩沖液高濃度快速試劑盒1,500 U 
7,500 U.10 U /μL2X快速連接緩沖液，
10X T4 DNA連接酶緩沖液

10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mMMgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.

10X T4 DNA連接酶緩沖液由500mM Tris-HCl，100mM MgCl 2,50mM二硫蘇糖醇，10mMATP，pH 7.6（25℃）組成。

2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mMMgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.

2X快速連接緩沖液由132mM Tris-HCl，20mM MgCl 2,2mM二硫蘇糖醇，2mM ATP，15％PEG，pH7.6（25℃）組成。

Figure 1:  Purity
The purity of the T4 DNA ligase is equal or greater than 95% asjudged by SDS-polyacrylamide gel electrophoresis with Coomassieblue staining.  Molecular weight markers are in Lane 1. T4 DNA ligase was loaded at 5 units (Lane 2) nd 10 units (Lane3).
圖1：純度
通過(guò)用考馬斯藍(lán)染色的SDS-聚丙烯酰胺凝膠電泳判斷，T4 DNA連接酶的純度等于或大于95％。分子量標(biāo)記在泳道1中.T4DNA連接酶以5個(gè)單位（泳道2）和10個(gè)單位（泳道3）加載。

Figure 2:  Exonuclease andEndonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonucleaseactivities, as judged by agarose gel electrophoresis followingincubation of 10 units of enzyme for 16 hours at 37 °C with 1 μg ofHindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA(Lanes 5 and 6).  Molecular weight markers are in Lanes 1 and2.

圖2：   核酸外切酶和核酸內(nèi)切酶活性測(cè)定
T4DNA連接酶不含外源和內(nèi)切核酸酶活性，可通過(guò)瓊脂糖凝膠電泳判斷，將10單位酶在37℃下與1μgHindⅢ消化后孵育16小時(shí)λDNA（泳道3和4）和超螺旋pUC19DNA（泳道5和6）。分子量標(biāo)記在泳道1和2中。
Unit Definition: One Weiss Unit is defined as the amount ofenzyme required to convert 1 nmol of 32P-labeled inorganicpyrophosphate into Norit adsorbable material in 20 minutes at 37°C, using specified reaction conditions. Note: 1 Weiss Unit isapproximately 67 cohesive end units.

Source: A recombinant E. coli strain carrying thecloned T4 DNA Ligase gene.

單位定義： 一個(gè)Weiss單位定義為使用特定的反應(yīng)條件，在37℃下在20分鐘內(nèi)將1nmol32P標(biāo)記的無(wú)機(jī)焦磷酸鹽轉(zhuǎn)化為Norit可吸附材料所需的酶量。注意：1 Weiss Unit約有67個(gè)粘性末端單元。

來(lái)源：   攜帶克隆的T4 DNA連接酶基因的重組  大腸桿菌菌株。

Contact your local distributor for pricingProductDescriptionSizeCat. No.NxGen? T4 DNA Ligase (Low Concentration)
1,500 U30241-1
7,500 U30241-2NxGen? T4 DNA Ligase (High Concentration)
1,500 U30243-1
7,500 U30243-2產(chǎn)品描述尺寸貨號(hào)NxGen?T4DNA連接酶（低濃度）
1,500 U.30241-1
7,500 U.30241-2NxGen?T4DNA連接酶（高濃度）
1,500 U.30243-1
7,500 U.30243-2ORDER INFORMATION
Storage buffer: T4 DNA Ligase is supplied in 10 mMTris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.1%TritonX-100, 50% glycerol, pH 7.5 @ 25 °C.

訂單信息
儲(chǔ)存緩沖液：  T4 DNA連接酶以10mM Tris-HCl，50mMKCl，1mM二硫蘇糖醇，0.1mM EDTA，0.1％Triton X-100,50％甘油，pH7.5在25℃下提供。

對(duì)此產(chǎn)品有興趣，可聯(lián)系中北林格獲取更多詳細(xì)信息。</p>