CAS12236-82-7
EINECS235-465-0化學(xué)式C29H20ClN7O11S3
分子量774.16溶解度H2O: 可溶存儲(chǔ)條件2-8°C穩(wěn)定性穩(wěn)定。與強(qiáng)氧化劑不相容。外觀鹽水懸浮液顏色dark blue體外研究Reactive Blue 2 is used as an ATP receptor antagonist and inducesCa 2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca 2+response to histamine that is linked to the PLC cascade. ReactiveBlue 2 may activate the PLC cascade in an extracellular Ca 2+-dependent manner and induce Ca 2+ oscillations. The application ofReactive Blue 2 increases K + secretion in a dose-dependent manner,and this increase is characterized as a peak followed by a partialrelaxation to a steady-state. Reactive Blue 2 has antagonisticactivities at P2Y4, and the antagonist potency at P2Y4 paralleledthe potency of K+ secretion.The anthra dye reactive blue 2is found to be a potent inhibitor of a protein kinase isolated andpurified from thylakoids. The mode of inhibition is noncompetitive,with a K i of 8 μM for the membrane-bound kinase, and 6 microM forthe purified kinase. The inhibitor does not modify the substratepreference of the endogenous kinase and could be removed from themembrane by washing. Reactive blue 2 selectively inhibits responsesmediated via the P2ypurinoceptor, at least within a limitedconcentration range. In preparations where the tone has been raisedwith noradrenaline, ATP and 2-methylthio ATP, but not α,β-methyleneATP, produce relaxations of the vessel. These relaxations areinhibited in the presence of reactive blue 2. Reactive blue 2, atconcentrations of 0.3-10 μM blocks the ATP-induced oscillation in aconcentration-dependent manner.