This isotopically labeled “heavy”tag can be used in parallel to the unlabeled“light” analog (See: UV CleavableBiotin-Azide) to screen protein adduction by identifying hitsas those with a mass difference of 6 Da.

Highlights:

Biotin conjugate can be subse visualized by streptavidinWesternues and/or enriched using streptavidin purificationmethods

Captured target can be released under mild photolysis conditions(365 nm) and results in a significant decrease of background signaldue to non-specifically bound proteins

參數.

示意圖.

參考文獻/References
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2. A Chemoproteomic Platform to Assess Bioactivation Potential ofDrugs. Sun, R.; Shi, F.; Liu, K.; Fu, L.; Tian, C.; Yang, Yong;Tallman, K. A.; Porter, N. A.; Yang, J. Chemical Research inToxicology 2017, 30(10), 1797.
3. Fu L, Liu K, He J, Tian C, Yu X, Yang J. Direct ProteomicMapping of Cysteine Persulfidation. Antioxid Redox Signal. 2020 Nov20;33(15):1061-1076. Viewarticle
4. Meng J, Fu L, Liu K, Tian C, Wu Z, Jung Y, Ferreira RB, CarrollKS, Blackwell TK, Yang J. Global profiling of distinct cysteineredox forms reveals wide-ranging redox regulation in C. elegans.Nat Commun. 2021 Mar 3;12(1):1415. Viewarticle